NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM301248 Query DataSets for GSM301248
Status Public on Jun 28, 2008
Title fresh spleen IKDC 1
Sample type RNA
 
Source name fresh spleen IKDC
Organism Mus musculus
Characteristics Balb/c mice
6-8 week old
Treatment protocol freshly isolated from organs by cell-sprting
Extracted molecule total RNA
Extraction protocol TRIZOL and Rneasy total RNA extraction following manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip murine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix 7G GeneChip scanner
Description gene expression data from spleen IKDC
Data processing The data were analyzed with GeneChip operating software version 1.4.
 
Submission date Jun 27, 2008
Last update date Aug 28, 2018
Contact name Franck Housseau
E-mail(s) [email protected]
Phone 410-614-0553
Fax 410-614-0549
Organization name Johns Hopkins University
Department Oncology
Lab Immunology CRB-I 424
Street address 1650 Orleans Street
City Baltimore
State/province MD
ZIP/Postal code 21231
Country USA
 
Platform ID GPL1261
Series (1)
GSE11918 Molecular characterization of murine IFN producing killer dendritic cells
Relations
Reanalyzed by GSE45704
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 296.787 P 0.000445901
AFFX-BioB-M_at 339.354 P 4.42873e-05
AFFX-BioB-3_at 230.887 P 4.42873e-05
AFFX-BioC-5_at 628.496 P 7.00668e-05
AFFX-BioC-3_at 484.323 P 4.42873e-05
AFFX-BioDn-5_at 639.423 P 4.42873e-05
AFFX-BioDn-3_at 2239.96 P 7.00668e-05
AFFX-CreX-5_at 6202.85 P 5.16732e-05
AFFX-CreX-3_at 6430.45 P 4.42873e-05
AFFX-DapX-5_at 10.3525 P 0.0219019
AFFX-DapX-M_at 3.50563 A 0.5
AFFX-DapX-3_at 1.29458 A 0.834139
AFFX-LysX-5_at 2.73651 A 0.60308
AFFX-LysX-M_at 4.2495 A 0.749204
AFFX-LysX-3_at 6.19236 A 0.195246
AFFX-PheX-5_at 0.544675 A 0.932322
AFFX-PheX-M_at 1.15154 A 0.794268
AFFX-PheX-3_at 12.979 A 0.227636
AFFX-ThrX-5_at 2.34994 A 0.672921
AFFX-ThrX-M_at 1.32136 A 0.48511

Total number of rows: 45101

Table truncated, full table size 1380 Kbytes.




Supplementary file Size Download File type/resource
GSM301248.CEL.gz 3.9 Mb (ftp)(http) CEL
GSM301248.CHP.gz 244.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap