|
| Status |
Public on Jul 27, 2008 |
| Title |
Math2 3 |
| Sample type |
RNA |
| |
|
| Source name |
Rat brain
|
| Organism |
Rattus norvegicus |
| Characteristics |
Cerebral cortices from 19-day-old fetal SD rats (E19)
|
| Biomaterial provider |
Clea Japan, Tokyo, Japan
|
| Treatment protocol |
Using TransFectin transfection reagent , we co-transfected cortical cells cultured in a 10-cm dish with 13.3 µg of Math2 expression vector or control vector and 2.7 µg of low-affinity nerve growth factor receptor (LNGFR) expression vector that encoded a cytoplasmically truncated cell surface marker. After incubation for 24 h, the cells were separated with a MACSelect LNGFR Transfected Cell Selection Kit according to the manufacturer's protocol.
|
| Growth protocol |
The culture medium used was Eagle MEM supplemented with 10% fetal calf serum; 5 % glucose ; 2 mM L-glutamine ; and 0.02mg/ml gentamycin .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After separation of the transfected cultured cortical cells, total RNA was extracted with Isogen reagent .
|
| Label |
Biotin
|
| Label protocol |
50 ng of total RNA was used for cDNA synthesis with a Two-Cycle Target Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45ºC on Affymetrix GeneChip Rat Genome 230 2.0 array.
|
| Scan protocol |
Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
|
| Description |
Primary cortical cells were transfected with control or math2 vector
|
| Data processing |
Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
|
| |
|
| Submission date |
Jun 19, 2008 |
| Last update date |
Jul 27, 2008 |
| Contact name |
Kou Takahashi |
| E-mail(s) |
[email protected]
|
| Organization name |
NCNP
|
| Street address |
4-1-1 Ogawahigashimachi, Kodaira
|
| City |
Tokyo |
| ZIP/Postal code |
187-8553 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11829 |
Prg1 is regulated by the basic helix-loop-helix transcription factor Math2 |
|
