|
| Status |
Public on Jun 12, 2008 |
| Title |
litter 1, P14 V1 Litter 1B |
| Sample type |
RNA |
| |
|
| Source name |
Mouse visual cortex P14 litter 1
|
| Organism |
Mus musculus |
| Characteristics |
129/SvEv, P14, mixed gender
|
| Growth protocol |
standard rearing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MGU74v2 ABC genechips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data mouse visual cortex
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 12, 2008 |
| Last update date |
Jun 27, 2008 |
| Contact name |
alvin lyckman |
| E-mail(s) |
[email protected]
|
| Organization name |
St Eliz Med Ctr
|
| Street address |
736 Cambridge St
|
| City |
Brighton |
| State/province |
MA |
| ZIP/Postal code |
02135 |
| Country |
USA |
| |
|
| Platform ID |
GPL82 |
| Series (1) |
| GSE11764 |
Expression data from postnatal day (P) 14, 28, 60 mouse visual cortex (V1) |
|
