|
| Status |
Public on Oct 08, 2008 |
| Title |
LWD1 and LWD2 in Arabidopsis photoperiod regulation (lwd1lwd2 rep 2) |
| Sample type |
RNA |
| |
|
| Source name |
lwd1lwd2 rep 2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ground tissues of 31-d-old wild-type Arabidopsis grown under 12 h L/12 h D were harvested at ZT5~9.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pine tree extraction method
Chang S, Puryear J, Cainey J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter 11: 113-116
|
| Label |
biotin
|
| Label protocol |
Ten microgram of total RNA was used for cDNA synthesis, labeled by in vitro transcription, followed by fragmentation according to the manufacturer’s suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix).
|
| |
|
| Hybridization protocol |
Eleven microgram labeled samples were hybridized to the ATH1 Genome Array at 45℃ for 16.5 hr. Washing and staining involved Fluidic Station-450 and the ATH1 Genome
|
| Scan protocol |
Array was scanned with use of the Affymetrix GeneChip Scanner 7G.
|
| Description |
This experiment was conducted to reveal genes differentially expressed between wild-type Arabidopsis and lwd1lwd2 double mutant at ZT5-9.
|
| Data processing |
The results were quantified and analyzed by use of MicroArray Suite 5.0 software (Affymetrix Inc.).
|
| |
|
| Submission date |
Jun 12, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shu-Hsing Wu |
| E-mail(s) |
[email protected]
|
| Phone |
886-2-27871178
|
| Fax |
886-2-27871178
|
| Organization name |
Institute of Plant and Microbial Biology, Academia Sinica
|
| Street address |
128, Sec 2, Academia Road
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE11762 |
LWD1 and LWD2 in Arabidopsis photoperiod regulation |
|
| Relations |
| Reanalyzed by |
GSE119083 |
