|
| Status |
Public on May 29, 2008 |
| Title |
HSC-Evi1-KO-1 |
| Sample type |
RNA |
| |
|
| Source name |
hematopoietic stem cells transduced with Cre
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6
Gender:male
Age:8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from sorted cells using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cDNA probes were prepared using the NuGen Ovation Biotin labeling system (NuGEN Technologies, Inc.), according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, Biotin-labeled cDNA was hybridized to the Mouse Genome 430 2.0 Array® (Affymetrix®) for 16 h at 45°C as recommended by the manufacturer. Washing was performed using an automated fluidics workstation, and the array was immediately scanned with GeneChip Scanner 3000 7G.
|
| Scan protocol |
The array was scanned with GeneChip Scanner 3000 7G.
|
| Description |
Lineage(-), Sca-1(+), c-Kit(+) cells derived from Evi-1(flox/flox) mice were transduced with GFP (control) or Cre-GFP expressing retroviruses. Total RNA was prepared from sorted GFP(+) cells.
|
| Data processing |
Expression data were extracted from image files produced on Affymetrix® GeneChip Operating Software 1.0® (GCOS®).
|
| |
|
| Submission date |
May 26, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Susumu Goyama |
| E-mail(s) |
[email protected]
|
| Organization name |
Tokyo University
|
| Department |
Hematology
|
| Street address |
7-3-1 Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11557 |
Effect of Evi-1 deletion in hematopoietic stem cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
