|
| Status |
Public on Sep 01, 2008 |
| Title |
root - 08% oxygen - 2h - F |
| Sample type |
RNA |
| |
|
| Source name |
root
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype col2
|
| Biomaterial provider |
Max Planck Insitute for Molecular Plant Physiology
|
| Treatment protocol |
Ten days after germination, seedlings were transferred to continuous darkness and a stream of premixed air containing 8% oxygen , 350 ppm CO2, and N2 was blown over the plates. Oxygen concentrations within the plates were regularly controlled to ensure precise regulation of the actual oxygen concentration around the roots. Roots were harvested in liquid nitrogen after 2 hours of each oxygen treatment.
|
| Growth protocol |
Arabidopsis thaliana ecotype col2 seedlings were grown in a 12h light / 12 h dark cycle (day and night temperature was 22°C) on vertical agar plates supplemented with half-strength Murashige and Skoog (MS) medium (Duchefa Biochemie B.V., Haarlem, the Netherlands) supplemented with 1% sucrose. The roots of the plants were growing on the surface of the agar, thus avoiding restricted oxygen diffusion towards the roots by the agar medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, according to the protocol recommended by the supplier (GibcoBRL Life Technologies)
|
| Label |
biotin
|
| Label protocol |
10ug of total RNA was biotin labelled
|
| |
|
| Hybridization protocol |
standard affymetrix protocol
|
| Scan protocol |
standard affymetrix protocol
|
| Description |
roots of 2 weeks old seedlings grown on vertical agar plates
|
| Data processing |
MAS5
|
| |
|
| Submission date |
May 24, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joost T. van Dongen |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0)331 5678353
|
| URL |
http://www.mpimp-golm.mpg.de
|
| Organization name |
Max Planck Institute of Molecular Plant Physiology
|
| Street address |
Am Muehlenberg 1
|
| City |
Potsdam |
| ZIP/Postal code |
D-14476 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE11558 |
transcript profiling of the adaptive response to decreases in oxygen concentration in the roots of Arabidopsis plants |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
