|
| Status |
Public on Sep 10, 2018 |
| Title |
D740H |
| Sample type |
SRA |
| |
|
| Source name |
Homo sapiens_Hypoxia
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Hypoxia
cell type: iPSC-Cardiomyocytes (iPSC-CM)
|
| Treatment protocol |
Normoxia (control) vs. 24hr Hypoxia
|
| Growth protocol |
CHIR 8uM x 2d + IWR 5uM x 2d + Glucose starvation on day 12 for 4 days. Cells were collected on day 30 of differentiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trisol/ Qiagen RNA extract kit
Ion Total RNA Seq Kit v2 with ERCC RNA Spike-in Mix 1 added
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Cufflinks
Cuffdiff
Genome_build: hg19/rheMac8
Supplementary_files_format_and_content: Gene-level differential expression. Tests differences in the summed FPKM of transcripts sharing each gene_id
|
| |
|
| Submission date |
Jan 02, 2018 |
| Last update date |
Sep 10, 2018 |
| Contact name |
Lei Tian |
| E-mail(s) |
[email protected]
|
| Phone |
6502388262
|
| Organization name |
Stanford's Postdoctoral Scholar programs
|
| Street address |
1215 Welch Rd
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE108676 |
Rhesus iPSC-CMs response to anoxia, comparing with human iPSC-CMs |
|
| Relations |
| BioSample |
SAMN08285280 |
| SRA |
SRX3526960 |
