|
| Status |
Public on Jun 19, 2018 |
| Title |
COS-7_CGG_TRA1 |
| Sample type |
RNA |
| |
|
| Source name |
COS-7_CGG_TRA
|
| Organism |
Chlorocebus sabaeus |
| Characteristics |
tissue source: kidney
cell line: COS-7
condition: transfected with CGG expansion at the 5'UTR FMR1 (60X) and with overexpression of TRA2A (cond5)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following Qiagen protocol including DNAse treatment
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA from each sample, isolated from COS-7 cells under different experimental conditions was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Plus protocol
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix Human Clariom D array using a Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Description |
CGG_TRA1_(Clariom_D_Human).sst-rma-gene-full.chp Signal
|
| Data processing |
The data were normalized using the SST-RMA algorithm and analyzed in the Transcriptome Analysis Console (TAC, Affymetrix)
The 'RMA_SST_ExonNormalization.txt' contains Exon log2 SST-RMA data
|
| |
|
| Submission date |
Dec 13, 2017 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE108007 |
The scaffolding role of RNA in ribonucleoprotein complexes: TRA2A recruitment in FXTAS disease |
|
