|
| Status |
Public on May 02, 2008 |
| Title |
Met_LymphNode_E_11 LN |
| Sample type |
RNA |
| |
|
| Source name |
Laser Captured Metastastic Lymph Node from E treated mouse #11
|
| Organism |
Mus musculus |
| Characteristics |
Strain: nu/nu Gender: female Age: 20 weeks
Tissue:breast cancer metastatic lymph node
|
| Treatment protocol |
Estrogen pellets implanted in mice, removed for 1 week for Estrogen withdrawal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Laser Capture was performed on frozen sections. After laser capture of cells, RNA PCR amplified.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human X3P Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent Bioanalyzer 2100
|
| Description |
Human Breast Cancer Cells grown in Mouse
|
| Data processing |
Data analyzed with Partek, imported and RMA normalized
|
| |
|
| Submission date |
May 01, 2008 |
| Last update date |
May 01, 2008 |
| Contact name |
Wendy Dye |
| Organization name |
UCDHSC
|
| Street address |
12801 E. 17th Ave
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE11317 |
Estrogen insensitivity in a model of estrogen receptor positive breast cancer lymph node metastasis. |
|
