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| Status |
Public on Nov 30, 2020 |
| Title |
m21_L_H3K4Me3-1 |
| Sample type |
SRA |
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| Source name |
Rat brain tissues
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| Organism |
Rattus norvegicus |
| Characteristics |
tissue: ipsilateral brain
age: 21 mon old
agent: none
chip antibody: H3K4Me3(Millipore,17-614)
disease: intracerebral hemorrhage
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated cell and histone-DNA complexes were isolated with antibody.
he libraries were prepared following the manufacturer's instructions （ThruPLEX DNA-seq 48S Kit, R400427）and applied to Illumina NextSeq 500 system for 150nt pair-end sequencing by ABlife Inc.
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
the ipsilateral brain tissue of intracerebral hemorrhage rats
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| Data processing |
Illumina Casava1.9 software used for basecalling.
3' adapter (end1:AGATCGGAAGAGC;end2:AGATCGGAAGAGC) trimmed with cutadapt
Reads were mapped using bowtie2 -N 1. Uniq mapped reads were remained.
macs14 was used to peakcalling with default parameters
homer was used to find motifs with options -len 5,6,7,8,9,10,11,12
Genome_build: Rattus norvegicus 6.0
Supplementary_files_format_and_content: peaks
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| Submission date |
Nov 15, 2017 |
| Last update date |
Nov 30, 2020 |
| Contact name |
dong chen |
| Organization name |
ablife
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| Street address |
GaoXin 2nd Road
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| City |
wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430064 |
| Country |
China |
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| Platform ID |
GPL20084 |
| Series (2) |
| GSE106951 |
High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats. [ChIP-Seq] |
| GSE106952 |
High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats. |
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| Relations |
| BioSample |
SAMN08031709 |
| SRA |
SRX3398096 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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