|
| Status |
Public on Nov 04, 2008 |
| Title |
PPV deletion mutant-Arabidopsis protoplasts-12hpt-used as control rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis protoplasts transfected with PPV deletion mutant at 12 hpt; used as control
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis protoplasts transfected with PPV deletion mutant at 12 hpt; used as control.
|
| Biomaterial provider |
Arabidopsis Protoplasts
|
| Treatment protocol |
Protoplasts transfected with PPV infection
|
| Growth protocol |
Arabidopsis grown at standard green house conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent to extract total RNA
|
| Label |
Single color
|
| Label protocol |
As per Affymetrix protocol
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol
|
| Scan protocol |
As per Affymetrix protocol
|
| Description |
Labelling, hybridization, and detection of hybridization signals were performed as per Affymetrix standard protocol
|
| Data processing |
Hybridization signals obtained from the scanned array images were quantified using MAS5.0 expression analysis algorithm, implemented in the Affymetrix GCOS (Gene Chip Operating) software version 1.2. Raw MAS5.0 data files obtained from scanned array images were imported into GeneSpring microarray analysis software version 7.3.1 (Silicon Genetics, Redwood City, CA, USA).
|
| |
|
| Submission date |
Apr 23, 2008 |
| Last update date |
Nov 04, 2008 |
| Contact name |
Mohan babu |
| E-mail(s) |
[email protected]
|
| Phone |
5194571470
|
| Fax |
5194573997
|
| Organization name |
AAFC
|
| Department |
Virology
|
| Lab |
Molecular Virology
|
| Street address |
1391 Sandford
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N5V 4T3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE11217 |
Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection |
|
