|
| Status |
Public on Jun 01, 2008 |
| Title |
A2B5+ biological replicate-2 |
| Sample type |
RNA |
| |
|
| Source name |
A2B5+ oligodendrocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
Fluorescent activated cell sorted A2B5+ oligodendrocytes isolated from post-natal day 7 rat whole brain
|
| Biomaterial provider |
Charles River
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy-Qiagen
|
| Label |
biotin
|
| Label protocol |
Affymetrix small sample labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocols were followed using the hybridization oven 640, and washing with the fluidics station 450
|
| Scan protocol |
The microarrays were scanned with a genechip scanner 3000 7G
|
| Description |
Oligodendrocytes were FAC sorted using cell surface expression of A2B5 and GALC to purifiy oligodendrocyte progenitors (A2B5+) and oligodendrocytes (GALC+) from postnatal day rat whole brains.
|
| Data processing |
Cel files were imported into Genespring for preprocessing using RMA, and normalization using a global per-chip and per-gene normalization strategy
|
| |
|
| Submission date |
Apr 20, 2008 |
| Last update date |
Oct 10, 2023 |
| Contact name |
Joe A Nielsen |
| E-mail(s) |
[email protected]
|
| Phone |
301-402-7350
|
| Organization name |
NIH/NINDS
|
| Lab |
Section of Develpmental Genetics
|
| Street address |
49 Convent Drive BD49,5A75
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4479 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11218 |
Identification of Dynamically Regulated MicroRNA and mRNA Networks in Oligodendrocytes |
|
| Relations |
| Reanalyzed by |
GSE245032 |
