|
| Status |
Public on Nov 24, 2008 |
| Title |
WTGR(-)::2 |
| Sample type |
RNA |
| |
|
| Source name |
WT Glucocorticoid receptor alpha treated for 0 hr, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
Human osteosarcoma, female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen) was used, according to manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Cells were grown in DMEM-F12 medium, supplemented with 10% Fetal Bovine Serum. 48 hour before treatments, cells were placed in DMEM-F12 medium supplemented with 10% charcoal-dextran-treated FBS. Cells were not treated or were treated with 100 nM dexamethasone for 6 hr.
|
| Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise. The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
|
| |
|
| Submission date |
Apr 17, 2008 |
| Last update date |
Jan 05, 2012 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE11205 |
Glycogen Synthase Kinase-3-beta Regulates Glucocorticoid Signaling by Phosphorylating the Glucocorticoid Receptor |
|
