|
| Status |
Public on Mar 19, 2022 |
| Title |
D4_KO_2 |
| Sample type |
SRA |
| |
|
| Source name |
mouse embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129
genotype: Hbxip-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA is extracted from cells using RNeasy Mini Kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
LIF withdrawal for 96h
|
| Data processing |
Basecalls performed using the software Zebra call
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using Bowtie2.
Fragments Per Kilobase per Million is calculated to represent the gene expression level
Genome_build: mm8
Supplementary_files_format_and_content: tab-delimited FPKM files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Sep 26, 2017 |
| Last update date |
Mar 20, 2022 |
| Contact name |
Ni Peiling |
| E-mail(s) |
[email protected]
|
| Phone |
13752270380
|
| Organization name |
nankai university
|
| Street address |
Weijin Road
|
| City |
Tianjin |
| ZIP/Postal code |
300071 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE104242 |
Transcriptome profiling of WT and Hbxip-KO E14 ESCs in pluripotent condition and during differentiation |
|
| Relations |
| BioSample |
SAMN07701820 |
| SRA |
SRX3216165 |
