|
| Status |
Public on Sep 30, 2020 |
| Title |
ZC3h12d-KO |
| Sample type |
RNA |
| |
|
| Source name |
spleen, ZC3h12dKO, TCM-stimulated
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: ZC3h12d KO
tissue: spleen
gender: male
treatment: tumor-conditioned medium (TCM)
|
| Treatment protocol |
ZC3h12d male mice were i.p. injected with LTCM at 3 tiles and spleens were collected.
|
| Growth protocol |
Whole spleens were collected and reserved at -80 degrees until total RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT Terminal Labeling and Control Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Mouse Transcriptome Array 1.0, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner.
|
| Description |
Male mouse spleen stimulated TCM.
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix Power Tools 1.15 software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Sep 19, 2017 |
| Last update date |
Sep 30, 2020 |
| Contact name |
Sachie Hiratsuka |
| E-mail(s) |
[email protected]
|
| Phone |
81-3-5269-7417
|
| Organization name |
Tokyo Women's Medical University
|
| Street address |
8-1 Kawada-cho Shinjyuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
162-8666 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
|
