|
| Status |
Public on Apr 30, 2019 |
| Title |
pl2.IL8, 1891, Cardia, 12 mo |
| Sample type |
RNA |
| |
|
| Source name |
mouse cardia with IL-1b and IL-8 overexpression
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cardia and forestomach
genotype: IL-1b and IL-8 overexpression
timepoint: 12 months
diet: control
concentration (µg/µl): 0.1
volume (µl): 4
|
| Treatment protocol |
Mice were allowed a standard chow diet from birth until weaning, and water ad libitum. Following weaning and genotyping, between 6 and 8 weeks of age, mice were assigned high fat diet or remained on the chow diet.
|
| Growth protocol |
Mice were maintained up to 12 months age in accordance with the German Animal Welfare and Ethical Guidelines of the Klinikum rechts der Isar, TUM, Munich, Germany up to
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After sacrificing mice, total RNA from cardia and forestomach tissues were extracted by TRIzol Reagen (Inventrogen) according to the manufacture’s protocol.
|
| Label |
Biotin
|
| Label protocol |
Total RNA samples (>50ng) were amplified and labelled.
|
| |
|
| Hybridization protocol |
Hybridization of AffymetrixMouse Gene2.1 ST arrays was performed as recommended by the manufacturer
|
| Scan protocol |
The microarrays were washed and stained in an Affymetrix Fluidics station and scanned in a GC3000 scanner with G7 update, as recommended by Affymetrix
|
| Description |
Gene expression data from mouse cardia with IL-1b and IL-8 overexpression
pl2.IL8, Cardia
1CM
|
| Data processing |
Signal summarization was done in Partek Genomics Suite using the RMA algorithm
|
| |
|
| Submission date |
Sep 07, 2017 |
| Last update date |
Apr 30, 2019 |
| Contact name |
Michael Quante |
| E-mail(s) |
[email protected]
|
| Organization name |
Technische Universität München
|
| Department |
II Medizinische Klinik
|
| Street address |
Ismaninger Str 22
|
| City |
München |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE103616 |
High fat diet accelerates Barrett esophageal carcinogenesis by inducing a distinct IL-8 dependent immune reaction through alteration of the microbiome |
|
