|
| Status |
Public on Apr 30, 2008 |
| Title |
Wildtype, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
lox95 control mouse
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: B6.Pdss2loxP/loxP
Age: 140-day-old male
40 mg liver used for RNA extraction
|
| Treatment protocol |
No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
|
| Growth protocol |
Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
|
| Description |
Gene expression data from wildtype M. musculus
|
| Data processing |
Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
|
| |
|
| Submission date |
Mar 20, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Eric F. Rappaport |
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Abramson Research Center
|
| Lab |
Room 906
|
| Street address |
3516 Civinc Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10904 |
Expression data from wildtype and alb/cre liver-conditional Pdss2 knockout mutant mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
