|
| Status |
Public on Dec 05, 2004 |
| Title |
Pool Ts1Cje_P15_hyb1 |
| Sample type |
RNA |
| |
|
| Source name |
Pool Ts1Cje_P15
|
| Organism |
Mus musculus |
| Characteristics |
ploidy: Ts1Cje (segmental trisomy of chromosome 16)
tissue: cerebellum
developmental stage: P15 postnatal stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen individual cerebellum
and treated with DNase using RNeasy Midi kit (Qiagen).
The quality of each RNA sample was then checked using
the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.,
Palo Alto, CA, USA).
|
| Label protocol |
An aliquot 20µg of total RNA from each pool was
converted to cDNA and then to biotinylated cRNA according to the Affymetrix procedures.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were hybridized to Affymetrix Murine Genome U74A version 2 microarrays (Affymetrix, Inc., Santa Clara, CA, USA) on the Curie Institute Affymetrix microarray platform (Paris, France), according to the Affymetrix procedures. Each pool was hybridized in duplicate on independent U74Av2 microarrays.
|
| Scan protocol |
According to standard Affymetrix procedures.
|
| Description |
Pool of total RNA from 3 Ts1Cje cerebella (segmental trisomy of chromosome 16) at P15 postnatal stage
Keywords = Down syndrome
Keywords = Chromosome 21
Keywords = Transcriptome
Keywords = Microarray
Keywords = Brain
Keywords = Cerebellum
Keywords = Development
|
| Data processing |
After hybridization, signal intensities were calculated using the Affymetrix GeneChip® software MAS 5.0
|
| |
|
| Submission date |
Jul 27, 2004 |
| Last update date |
Jun 24, 2009 |
| Contact name |
Luce Dauphinot |
| E-mail(s) |
[email protected]
|
| Phone |
33 1 57274518
|
| Organization name |
INSERM U1127-CNRS UMR7225-UPMC
|
| Department |
ICM
|
| Lab |
Alzheimer and Prions Disease team
|
| Street address |
47 boulevard de l'hôpital
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE1611 |
Transcriptome of Ts1Cje and euploids cerebellum |
|
