|
| Status |
Public on Jun 25, 2019 |
| Title |
18-Mbd3-KO-Mbd3a(deltaMBD)-LIF_minus |
| Sample type |
RNA |
| |
|
| Source name |
18-Mbd3-KO-Mbd3a(deltaMBD)-LIF minus
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: 129SV
cell-type: embryonic stem cells
|
| Treatment protocol |
Differentiation of ESCs were induced by withdrawal LIF for 12days.
|
| Growth protocol |
ESCs were culture under conventional ESC medium with LIF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared using RNeasy Kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT Plus Kit.
|
| |
|
| Hybridization protocol |
Affymetrix Standard WT Plus Protocol.
|
| Scan protocol |
AGCC Standard Protocol.
|
| Description |
cultured under conventional ESCs medium without LIF for 12days.
|
| Data processing |
The data was background-subtracted and normalized with the robust multi-array analysis (RMA; Irizarry et al. Nucleic Acids Res 31(4): e15, 2003) via R/Bioconductor 2.7 affy package using default analysis settings.
|
| |
|
| Submission date |
Aug 10, 2017 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Akihiko Okuda |
| E-mail(s) |
[email protected]
|
| Phone |
81-42-984-4787
|
| Organization name |
Saitama Medical University
|
| Department |
Research Center for Genomic Medicine
|
| Lab |
Division of Biomedical Sciences
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
3501241 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE102499 |
Identification of the coiled-coil domain as an essential element of MBD3 for preserving lineage commitment potential of ESCs |
|
