|
| Status |
Public on Jan 12, 2018 |
| Title |
MIX-A3,MIX rep2 |
| Sample type |
RNA |
| |
|
| Source name |
endometrium isolated from subcutaneous implantation of the 17-β estradiol (E2) pellet and high-fat diet mice
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
strain: C57BL/6
tissue: Endometrium
|
| Treatment protocol |
Mouse uterus was longitudinally dissected and scraped using the back of a scalpel into RNA Later on ice.
|
| Growth protocol |
Mice were matained in above four treatment for 3 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using miRNeasy Mini Kit and RNase-Free Dnase Set.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using GeneChip® WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Transcriptome Array 1.0 in GeneChip® Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G
|
| Description |
Gene expression data from ovariectomized mice endometrium(estrogen + high-fat diet)
|
| Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 01, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Xiaojun Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Obstetrics and Gynecology Hospital of Fudan University
|
| Street address |
No. 419, Fangxie Road
|
| City |
Shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE102103 |
Estrogen and high-fat diet induced alterations of endometrial transcriptome profile in ovariectomized C57BL/6 mice |
|
