|
| Status |
Public on Dec 19, 2017 |
| Title |
hip_ctrl_age_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, aged control
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: F344
age (mo): 21 months old
gender: male
tissue: hippocampus
treatment: AAV GFP ST
rin: 9.6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Rat Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000 7G scanner
|
| Description |
Gene expression data from aged viral vector control hippocampus, injected at age 18 months, assayed at 21 months (short term- 3 month- exposure)
|
| Data processing |
The data were analyzed with Partek software using the Robust Multi-Array (RMA) algorithm with default analysis settings
|
| |
|
| Submission date |
Jul 31, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
[email protected]
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE102054 |
FFK506-Binding Protein 12.6/1b, a negative regulator of [Ca2+], rescues memory and restores genomic regulation in the hippocampus of aging rats |
|
