|
| Status |
Public on Feb 05, 2018 |
| Title |
NC2 |
| Sample type |
SRA |
| |
|
| Source name |
Health donor_peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Health donor
tissue: Peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were isolated from Peripheral blood，and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Transcriptomic reads from the RNA-Seq experiments were mapped to a reference genome (mm10) using Bowtie.
Gene expression levels were quantified using the RSEM software package.
Differentially expressed genes were subsequently analyzed using the DAVID bioinformatics platformand IPA.
Genome_build: hg38
Supplementary_files_format_and_content: Processed data were txt format and all gene FPKM of each sample were contained in each txt file.
|
| |
|
| Submission date |
Jul 14, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Junli Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Health Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences
|
| Street address |
New Research Buliding,320 Yue Yang Road Shanghai, P.R.China
|
| City |
上海 |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE101437 |
Next Generation Sequencing Facilitates Quantitative Analysis of Health donors and SLE patients' PBMC Transcriptomes |
|
| Relations |
| BioSample |
SAMN07350924 |
| SRA |
SRX3005298 |
