|
Status |
Public on Feb 29, 2008 |
Title |
Agilent 244k Chip 2 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
JW DNA extracted from blood
|
Organism |
Homo sapiens |
Characteristics |
Blood from JW
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA extracted from blood using FlexiGene DNA kit from Qiagen
|
Label |
Cy5
|
Label protocol |
The labelling reaction was performed with Bioprime CGH labelling Module (Invitrogen) at 378C for 2 hours in the presence of Cy5-dCTP following standard Agilent protocal
|
|
|
Channel 2 |
Source name |
Reference DNA
|
Organism |
Homo sapiens |
Characteristics |
Blood from reference DNA, Coriell number NA10851
|
Biomaterial provider |
Coriell http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA10851
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA extracted from blood using FlexiGene DNA kit from Qiagen
|
Label |
Cy3
|
Label protocol |
The labelling reaction was performed with Bioprime CGH labelling Module (Invitrogen) at 378C for 2 hours in the presence of Cy3-dCTP (PerkinElmer) following standard Agilent protocal
|
|
|
|
Hybridization protocol |
Experimental and reference targets for each hybridization were purified, pooled and incubated with human Cot-1 DNA (Invitrogen) and Blocking Agent (Agilent Technologies, Inc.)
|
Scan protocol |
Slides were scanned into image files using an Axon microarray scanner (GenePix 4000B from Axon Instruments, Union City, CA, USA)
|
Description |
CNV data for JW (Agilent Human Genome CGH 244K Array)
|
Data processing |
Data was analyzed using the CGH Analytics software (Agilent Technologies, Inc.)
|
|
|
Submission date |
Feb 28, 2008 |
Last update date |
May 13, 2008 |
Contact name |
Omar Alvi |
E-mail(s) |
[email protected]
|
Organization name |
Baylor College of Medicine
|
Department |
Human Genome Sequencing Center
|
Street address |
1 Baylor Plaza
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL4544 |
Series (1) |
GSE10668 |
The Complete Genome of a Single Individual by Massively Parallel DNA Sequencing |
|
Data table header descriptions |
ID_REF |
|
VALUE |
log(REDsignal/GREENsignal) per feature (processed signals used). |
LogRatioError |
error of the log ratio calculated according to the error model chosen. |
PValueLogRatio |
Significance level of the Log Ratio computed for a feature. |
gProcessedSignal |
Dye-normalized signal after surrogate algorithm, green channel, used for computation of log ratio. |
rProcessedSignal |
Dye-normalized signal after surrogate algorithm, red channel, used for computation of log ratio. |