|
| Status |
Public on Feb 20, 2009 |
| Title |
control, biological rep10 |
| Sample type |
RNA |
| |
|
| Source name |
normal lung tissues, control, biological rep10
|
| Organism |
Homo sapiens |
| Characteristics |
Normal lung tissues obtained from the disease free margins with normal histology of lung cancer resection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNeasy cleanup was performed according to the manufacturers' instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA were prepared according to the standard Agilent protocol from 500ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.5 ug of cRNA were hybridized for 17 hr at 65C on Agilent 4x44k Whole Human Genome Array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Agilent Scanner protocol
|
| Description |
Lung tissue samples obtained from the University of Pittsburgh Health Sciences Tissue Bank. Gene expression data from a control lung.
|
| Data processing |
Agilent Feature Extraction
|
| |
|
| Submission date |
Feb 28, 2008 |
| Last update date |
Mar 26, 2008 |
| Contact name |
Kazuhisa Konishi |
| E-mail(s) |
[email protected]
|
| Phone |
412-647-3156
|
| Organization name |
University of Pittsburgh
|
| Department |
Pulmonry, Allergy, and Critical Care Medicine
|
| Lab |
Kaminski Lab
|
| Street address |
1212 Kaufmann Building 3471 Fifth Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE10667 |
MMP1 and MMP7 as potential peripheral blood biomarkers in Idiopathic Pulmonary Fibrosis |
|
