|
| Status |
Public on Oct 31, 2017 |
| Title |
JL08_20 |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted hWAT SVF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T-cells
experimental group: lean
|
| Treatment protocol |
no treatments
|
| Growth protocol |
fresh samples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
collagenase purification of adipocytes and SVF has been described previously in
|
| Label |
biotin
|
| Label protocol |
10 ng of total RNA were amplified in 4 cycles to prepare biotinylated ss-cDNA according to the Affymetrix WT Pico protocol
|
| |
|
| Hybridization protocol |
Fragmented cRNA were hybridized for 16 hr at 45C to Affymetrix Clariom D human arrays according to standard protocol. Washing and staing were performed using Affymetrix Fluidics Station 450.
|
| Scan protocol |
The arrays were scannned using an Affymetrix Gene Chip Scanner 3000.
|
| Data processing |
Generated CEL files were analyzed in Affymetrix Expression Console v. 1.4 using the SST-RMA method.
|
| |
|
| Submission date |
Jul 05, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Jurga Laurencikiene |
| Organization name |
Karolinska Institutet
|
| Street address |
Hälsovägen 7
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE100795 |
Transcriptome of specific cell types residing in human subcutaneous adipose tissue |
|
