|
| Status |
Public on Jul 11, 2017 |
| Title |
Pol II ChIP-seq siRNA control replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: non-targeting control siRNA
antibody: Pol II Active Motif 39097
cell line: HeLa
|
| Treatment protocol |
Cells were transfected with siRNAs and harvested for ChIP after 72 hours
|
| Growth protocol |
Cells were maintained in DMEM with 10% FBS, 5% CO2, at 37 degrees
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from crosslinked sheared chromatin subject to immunoprecipitation with the Pol II antibody
ChIP–seq samples were sequenced using the Illumina Solexa Hiseq 2000.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed by Illumina CASAVA 1.8.2
ChIP-seq reads were aligned to the hg19 genome using Bowtie v1.1.0
Genome_build: hg19
Supplementary_files_format_and_content: ChIP-seq wig files were generated using MACS-1.4.2
|
| |
|
| Submission date |
Jun 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiejun Shi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California Irvine
|
| Department |
School of Medicine
|
| Lab |
Li lab
|
| Street address |
5270 California Ave
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE99998 |
Acetylation on Histone H3 Lysine 9 Mediates a Switch from Transcription Initiation to Elongation |
|
| Relations |
| BioSample |
SAMN07229650 |
| SRA |
SRX2915875 |
