|
Status |
Public on Feb 14, 2008 |
Title |
rheumatoid arthritis 9252 |
Sample type |
RNA |
|
|
Source name |
RA patient
|
Organism |
Homo sapiens |
Characteristics |
Synovial fluid macrophages
|
Biomaterial provider |
HSS
|
Treatment protocol |
no treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Labeled cDNA was prepared with BioArray High Yield RNA Transcription Kit (Enzo Life Sciences, Farmingdale,NY)
|
|
|
Hybridization protocol |
hybridized to Affymetrix HG U95 Av2 GeneChip using Affymetrix fluidics station according to the instructions of the manufacturer
|
Scan protocol |
Affymetrix Gene-Array Scanner
|
Description |
Synovial fluid macrophages from individual RA donor
|
Data processing |
Affymetrix Microarray Suite MAS 5.0. Images were scaled to an average hybridization intensity of 250. Further data analysis was performed using GeneSpring software.
|
|
|
Submission date |
Feb 11, 2008 |
Last update date |
Mar 16, 2009 |
Contact name |
Taras Antoniv |
Organization name |
Hospital for Special Surgery
|
Department |
Research
|
Lab |
Lionel B. Ivashkiv, MD
|
Street address |
535 East 70th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL8300 |
Series (1) |
GSE10500 |
Gene expression in rheumatoid arthritis synovial macrophages |
|