|
| Status |
Public on Mar 01, 2008 |
| Title |
18.ko.hca.rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic ATF4 KO cortical neurons, HCA treated, replicate #3
|
| Organism |
Mus musculus |
| Characteristics |
15.5 dpc ATF4 KO embryonic neurons after oxidative stress induced by the glutamate analog homocysteate (HCA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction: Ambion RNAqeuous kit
Quality assessment: Agilent Bioanalyzer
|
| Label |
Biotin
|
| Label protocol |
According to Illumina protocol
|
| |
|
| Hybridization protocol |
According to Illumina protocol
|
| Scan protocol |
Illumina BeadScan
Image extraction: BeadStudio version 1.5.1.3, default parameters, no background subtraction
|
| Description |
1541554117_B: array designation within the raw data file linked to the Series record
|
| Data processing |
Raw data was analyzed using Bioconductor packages (www.bioconductor.org). Data was normalized using quantile normalization. Analysis of differential expression was performed using a linear model fitting (LIMMA package, Smyth 2005).
|
| |
|
| Submission date |
Feb 11, 2008 |
| Last update date |
Feb 12, 2008 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
[email protected]
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL6100 |
| Series (1) |
| GSE10470 |
Gene Expression Study in WT and ATF4 KO embryonic neurons |
|
