|
| Status |
Public on Jun 02, 2017 |
| Title |
Slc51a knockout mouse ileum, biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Ileal tissue Slc51a knockout mouse ileum
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ileal
genotype/variation: Slc51a knockout
strain: C57BL/6 background
gender: male
|
| Treatment protocol |
Mice described above were fasted for 4 hours, anesthetized with ketamine-xylazine, and then sacrificed by decapitation. Ileal tissue was collected and stored in liquid nitrogen until used to isolate RNA.
|
| Growth protocol |
Wild type and Slc51a knockout mice (males, C57BL/6 background; ~2 months of age)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed. The RNA was further purified using an Rneasy MinElute Cleanup Kit (Qiagen #74204)
|
| Label |
biotin
|
| Label protocol |
250 ng of purified total RNA was reverse transcribed and biotin labeled to produce biotynylated cRNA tagets according to the standard Affymetrix GeneAtlas 3'-IVT Express labeling protocol (GeneAtlas 3' IVT Expression Kit User Manual, P/N 702833 Rev. 4, Affymetrix).
|
| |
|
| Hybridization protocol |
6 ug of biotynylated cRNA was hybridized for 16 h at 45 °C with the Affymetrix GeneAtlas Mouse MG-430 PM Array Strips. Strips were washed and stained using the GeneAtlas Fluidics Station according to standard Affymetrix operating procedures (GeneAtlas™ System User’s Guide; P/N 08-0306 Rev A January 2010)
|
| Scan protocol |
Strips were scanned using the GeneAtlas Imager system.
|
| Description |
Gene expression data from mouse ileal tissue
|
| Data processing |
Fluidics Control, Scan control and data collection was performed using the GeneAtlas Instrument Control Software version 1.0.5.267. The raw data generated were normalized using the robust multi-array average (RMA) method.
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| |
|
| Submission date |
Jun 01, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Paul Dawson |
| E-mail(s) |
[email protected]
|
| Phone |
404-727-7083
|
| Organization name |
Emory University
|
| Department |
Division of Pediatric GI/Hepatology
|
| Lab |
E-263
|
| Street address |
1760 Haygood Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE99579 |
Expression data from ileum of adult male wild type and Slc51a knockout mice, where the basolateral membrane bile acid transporter subunit encoded by Slc51a is inactivated. |
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