|
| Status |
Public on Dec 21, 2017 |
| Title |
Male_LFD_Replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
colon epithelium from mice on the LFD
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
gender: male
tissue: colon epithelium
|
| Treatment protocol |
One week acclimation after shipment, mice were put on the HFD and LFD for 20 weeks. Colon epithelium was collected.
|
| Growth protocol |
Male and female C57Bl6 mice were purchased from JAX.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen Rneasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Two hundred and fifty nanograms (250 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
Five and one half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression data from colon epithelium
|
| Data processing |
Data was obtained using the GeneChip® Command Console software.
RMA
|
| |
|
| Submission date |
May 25, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE99327 |
Metabolic products of an obesity-associated microbiome impact host metabolism and disease risk by altering the epigenome |
|
