|
| Status |
Public on Apr 10, 2008 |
| Title |
MDA-MB435-3B2 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB435 transduced with lentiviral vector encoding SEMA 3B
|
| Organism |
Homo sapiens |
| Characteristics |
MDA-MB435 transduced with lentiviral vector encoding SEMA 3B, Melanoma-derived Cell Line
|
| Treatment protocol |
Cells were transduced with lentiviral vectors either encoding SEMA3B (“3B”) or a non-coding empty vector (“EV”).
|
| Growth protocol |
Cells were grown in standard medium supplemented with with L-glutamine and 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from tumor cell lines was isolated by using RNeasy Protect Mini Kit (Qiagen) according to the manufacturer instruction
|
| Label |
biotin
|
| Label protocol |
Five hundred ng of total RNA was reverse transcribed and amplified overnight with T7 RNA polymerase and labeled with biotin following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
1.5 microgram of biotin-labeled cRNA was hybridized to Illumina Human WG-6 BeadChips at 55 °C for 18hrs. BeadChips were incubated with Cy3 streptavidin and washed according to the manufacturer’s protocol.
|
| Scan protocol |
The hybridized BeadChips were scanned by Illumina BeadScan confocal scanner and analyzed by Illumina's BeadStudio version 1.5.1.3
|
| Description |
Cells expressing SEMA 3B
|
| Data processing |
Data were normalized with the Illumina Beadstudio software, using rank-invariant normalization
|
| |
|
| Submission date |
Feb 07, 2008 |
| Last update date |
Apr 10, 2008 |
| Contact name |
Enzo Medico |
| E-mail(s) |
[email protected]
|
| Phone |
+39-011-9933234
|
| Organization name |
Candiolo Cancer Institute, University of Torino
|
| Department |
Oncology
|
| Lab |
Laboratory of Oncogenomics
|
| Street address |
Strada Prov. 142, km 3,95
|
| City |
Candiolo |
| State/province |
TO |
| ZIP/Postal code |
10060 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6097 |
| Series (1) |
| GSE10431 |
The tumor suppressor Semaphorin 3B triggers a pro-metastatic program mediated by IL-8 and the tumor microenvironment |
|
