|
| Status |
Public on Nov 01, 2017 |
| Title |
SUDHL-4 100nM CPI203 6h |
| Sample type |
RNA |
| |
|
| Source name |
SUDHL-4 100nM CPI203
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DHL cell line SUDHL-4
agent: CPI203
|
| Treatment protocol |
DHL cells were treated with 100 nM CPI203 for 6h
|
| Growth protocol |
DHL cells at exponential phase were set at 1x10^6 cells/ml for the experiment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA
|
| |
|
| Hybridization protocol |
RNA samples were processed to Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the Gene Titan instrument.
|
| Description |
Double Hit Lymphoma cell line
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
Raw data were normalized using the Robust Multichip Analysis (RMA) algorithm implemented in the Expression Console Software v1.1 (Affymetrix).
|
| |
|
| Submission date |
May 15, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Juan Garcia Valero |
| E-mail(s) |
[email protected]
|
| Organization name |
IDIBAPS
|
| Department |
Oncology and haematology
|
| Lab |
Physiopathology and molecular bases in hematology
|
| Street address |
Rosselló, 153
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE98905 |
The BET bromodomain inhibitor CPI203 overcomes resistance to ABT-199 (venetoclax) by downregulation of BFL-1/A1 in in vitro and in vivo models of MYC+/BCL2+ double hit lymphoma |
|
