|
| Status |
Public on Feb 01, 2008 |
| Title |
WT Tibialis Anterior at 4 weeks biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Tibialis Anterior 4 weeks post birth
|
| Organism |
Mus musculus |
| Characteristics |
H6PDH WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from muscle of 4 weeks old altered in glucose and steroid metabolism
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and as quantile normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 31, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Gareth Lavery |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Birmingham
|
| Street address |
2nd Floor IBR
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10347 |
Gene expression data from Hexose-6-phosphate dehydrogenase knockout mouse muscle at 4 weeks |
|
| Relations |
| Reanalyzed by |
GSE119085 |
