|
| Status |
Public on Mar 31, 2008 |
| Title |
SNL+ GPN sc,Ipsi set B |
| Sample type |
RNA |
| |
|
| Source name |
Rat hemisected spinal cord, ipsilateral(ipsi) to the injury.
|
| Organism |
Rattus norvegicus |
| Characteristics |
rats from five experimental groups were sacrificed 19–21 days after surgery
|
| Treatment protocol |
Rats having the L5-L6 spinal nerves ligated (SNL) were performed, Sham-operation consisted of exposing the spinal nerves without ligation or manipulation. Naïve animals did not receive surgery.Rats were tested before and 12–14 days after surgery. Only SNL rats exhibiting a significantly reduced PWT in the ipsilateral limb were included in drug testing or gene expression studies (eight to twelve rats per group). Vehicle (0.9% saline) or gabapentin (GPN; 100 mg/kg dissolved in 0.9% saline) was administered intraperitoneally (i.p.) once a day for 7 days in a volume of 2 ml/kg. On the last (7th) treatment day (corresponding to 19–21 days post-surgery), rats were again assessed by PWT, in particular to confirm the reversal of neuropathic pain with GPN treatment.
|
| Growth protocol |
For gene expression analysis, rats from five experimental groups were sacrificed 19–21 days after surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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| |
|
| Submission date |
Jan 22, 2008 |
| Last update date |
Jan 22, 2008 |
| Contact name |
Jason Gang Jin |
| E-mail(s) |
[email protected]
|
| Phone |
732-220-9988
|
| Fax |
732-960-2355
|
| Organization name |
MaxyBio
|
| Street address |
675 US Highway
|
| City |
North Brunswick |
| ZIP/Postal code |
08902 |
| Country |
USA |
| |
|
| Platform ID |
GPL86 |
| Series (1) |
| GSE10238 |
Complement activation in the peripheral nervous system following the spinal nerve ligation model of neuropathic pain |
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