|
| Status |
Public on Sep 01, 2008 |
| Title |
Phloem exudate_3 |
| Sample type |
RNA |
| |
|
| Source name |
Phloem exudate of Arabidopsis
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Phloem exudate of adult leaves of early flowering Arabidopsis thaliana, ecotype Col-0 extracted by the EDTA-chelation technique.
|
| Biomaterial provider |
na
|
| Treatment protocol |
na
|
| Growth protocol |
Plants were cultivated under short day conditions (8 h light, 22 °C / 16 h darkness, 16°C) in a growth chamber (Percival Scientific, Inc. USA).
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
PolyA-RNA was extracted from phloem exudates using Oligo-dT25 Dynabeads (Dynal, Hamburg, Germany) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix protocol.
|
| Scan protocol |
Microarrays were scanned using the GCS3000 Gene Chip scanner (Affymetrix) and GCOS 1.4 software.
|
| Description |
Microarray analysis were conducted at The Microarray Facility, University of Tuebingen Germany. RNA of the sample was subjected to two rounds of linear amplification using Affymetrix “Two-Cycle cDNA synthesis kit” (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.
|
| Data processing |
Affymetrix MAS 5.0 software.
|
| |
|
| Submission date |
Jan 21, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Rosalia Deeken |
| E-mail(s) |
[email protected]
|
| Phone |
+49 931 8886121
|
| Fax |
+49 931 8886158
|
| URL |
http://www.botanik.uni-wuerzburg.de
|
| Organization name |
University of Wuerzburg, Julius-von-Sachs-Institute
|
| Department |
Department of Molecular Plant Physiology and Biophysics
|
| Lab |
Rainer Hedrich
|
| Street address |
Julius-von-Sachs-Platz 2
|
| City |
Wuerzburg |
| ZIP/Postal code |
97082 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10247 |
Transcriptome analysis of the Arabidopsis phloem |
|
| Relations |
| Reanalyzed by |
GSE119083 |
