|
| Status |
Public on Jan 31, 2008 |
| Title |
vector control cells, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
vector control cells, biological rep2
|
| Organism |
Homo sapiens |
| Characteristics |
MCF10A cells
|
| Growth protocol |
Standard MCF10A growth conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G System.
|
| Description |
MCF10A cells were infected with retrovirus constructs (vector or YAP) and puromycin was used to select for transduced cells. Cells were split and grown to ~60-75%% confluency at which point they were harvested for RNA.
|
| Data processing |
The data were analyzed with GeneChip Operating Software Ver. 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 17, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel Haber |
| Organization name |
Massachusetts General Hospital
|
| Department |
Cancer Center
|
| Street address |
Rm.7119, Bldg.149, 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10196 |
Identification of YAP target genes in MCF10A cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
