|
| Status |
Public on Apr 08, 2017 |
| Title |
Heat sensitive 15-rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
shoot meristem of heat sensitive broccoli, 15 degree C, replicate 2
|
| Organism |
Brassica oleracea var. italica |
| Characteristics |
tissue: shoot meristem
genotype: heat-sensitive
temperature: 15
|
| Growth protocol |
The heat-tolerant and heat-sensitive broccoli were grown in growth chambers in long day condition with 18 h light / 6 h dark. Seeds were germinated in vermiculite for 10 days and transplanted to 9 cm diameter pot in soil for 30 days at 22℃. The plants were then transferred to 15, 22, and 27℃, respectively, for 2 weeks and then extracted the total RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using QIAGEN RNeasy kit follwed by DNAse treatment. The RNA samples were further purified and concentrated by RNeasy MinElute Cleanup.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was produced with the Quick Amp Labeling Kit, one-color (Agilent Technologies) according the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization was produced with the Quick Amp Labeling Kit, one-color (Agilent Technologies) according the manufacturer’s instructions.
|
| Scan protocol |
Slides were scanned at 5 μm resolution with a Agilent Technologies Scanner G2505C scanner.
|
| Data processing |
An image analysis and normalization software is used to quantify signal and background intensity for each feature, substantially normalized the data by rank-consistency-filtering LOWESS method.
|
| |
|
| Submission date |
Apr 07, 2017 |
| Last update date |
Apr 08, 2017 |
| Contact name |
Chung-Wen Lin |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cheng Kung University
|
| Department |
Department of Life Sciences
|
| Street address |
No. 1, University Rd
|
| City |
Tainan City |
| ZIP/Postal code |
701 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL15739 |
| Series (1) |
| GSE97528 |
Genome-wide survey prior to head formation reveals new insights into the molecular basis of high temperature stress in tolerant and sensitive broccoli genotypes |
|
