|
| Status |
Public on Feb 13, 2018 |
| Title |
MDS1277 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
cohort: validation
disease state: MDS
tissue: Bone marrow
condition: Pre-treatment
age: 59.8
gender: Male
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by TriZol reagent according to manufacturer's instructions. One microgram of RNA from each sample was processed with the QIAGEN miRNeasy mini kit (QIAGEN, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified (in vitro transcription) and generate biotin labeled Single-stranded cDNA according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Affymetrix GeneChip Human Transcriptome Array 2.0 according to the manufacturer’s instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
lncRNA expression data from BM mononuclear cells
VALIDATION 16
|
| Data processing |
Expression values were processed with Signal Space Transformation (SST) and Robust Multichip Average method, which included log2 transformation and quantile normalization.
|
| |
|
| Submission date |
Mar 27, 2017 |
| Last update date |
Feb 13, 2018 |
| Contact name |
Chi-Yuan Yao |
| E-mail(s) |
[email protected]
|
| Phone |
+886-972651859
|
| Organization name |
National Taiwan University Hospital
|
| Street address |
No.7, Chung Shan S. Rd.
|
| City |
Taipei City |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE97064 |
Long noncoding RNA (lncRNA) expression data from human myelodysplastic syndromes (MDS) bone marrow mononuclear cells |
|
