|
| Status |
Public on Dec 20, 2007 |
| Title |
Mouse pituitary gland, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse pituitary gland
|
| Organism |
Mus musculus |
| Characteristics |
C57Bl6 male mice (10-12 weeks old)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Absolutely RNA microprep from Stratagene (CA). The total RNA quantity and quality was determined using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, DW) and the 2100 Bioanalyzer (Agilent, Waldbronn, Germany), respectively. Total RNA profiles of all tested samples were similar with sharp 18S and 28S rRNA peaks on a flat baseline.
|
| Label |
Biotin
|
| Label protocol |
Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). One ??g of total RNA was used to prepare biotinylated cRNA with IVT labeling kit (Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.5. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94??C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridised to mouse 430 2.0 arrays (Affymetrix) during 16h at 45??C.
|
| Scan protocol |
The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
|
| Description |
Mouse pituitary gland
|
| Data processing |
All image files were analysed using the affy library of the BioConductor project (no background adjustment, probe-level quantile normalization, and average difference summarization)
|
| |
|
| Submission date |
Dec 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Léon-Charles Tranchevent |
| E-mail(s) |
[email protected]
|
| Organization name |
Katholieke Universiteit Leuven
|
| Department |
Departement of Electrical Engineering
|
| Lab |
ESAT-SCD
|
| Street address |
Kasteelpark Arenberg 10
|
| City |
Leuven-Heverlee |
| ZIP/Postal code |
B-3001 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9954 |
Large-scale analysis of the mouse transcriptome |
|
| Relations |
| Reanalyzed by |
GSM595400 |
| Reanalyzed by |
GSE119085 |
