|
| Status |
Public on Feb 28, 2018 |
| Title |
MoDC-control-6h-1 |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte derived dendritic cell cultured without Lipiodol
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Monocyte-derived dendritic cell
|
| Treatment protocol |
Monocyte derived dendritic cells were cultured with or without Lipiodol for 6 hours.
|
| Growth protocol |
Dendritic cells (monocyte derived dendritic cells) were induced by culturing CD14 positive monocyte with GM-CSF and IL-4 for 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted by RNeasy mini kit® following manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
|
| |
|
| Hybridization protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
|
| Scan protocol |
We followed manufacturer's protocol, and used GeneChip® Scanner 3000 7G.
|
| Data processing |
Data acquisition and normalization (SST-RMA) were used Affymetrix® Expression Console™ Software.
|
| |
|
| Submission date |
Feb 28, 2017 |
| Last update date |
Feb 28, 2018 |
| Contact name |
Gentaro Izumi |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Tokyo
|
| Street address |
Senju-kotobukicho 6-15-1203
|
| City |
Adachi |
| State/province |
Tokyo |
| ZIP/Postal code |
120-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE95485 |
Effect of Lipiodol on Dendritic Cell |
|
