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Sample GSM2482093 Query DataSets for GSM2482093
Status Public on Jun 29, 2017
Title siSLC25A10 [212_siSLC25A10_C07]
Sample type RNA
 
Source name primary adipocyte culture, siSLC25A10
Organism Homo sapiens
Characteristics disease status: healthy, non-obese
tissue: abdominal subcutaneous white adipose tissue (WAT)
cell type: adipocytes
sirna: siSLC25A10
Treatment protocol In vitro differentiated adipocytes (day 10-12 post induction) were transfected with ON-TARGETplus SMARTpool small interfering RNAs (siRNAs) targeting KLF15, SLC25A10 or non-targeting siRNA pool at 40 nM final concentration (Dharmacon, Lafayette, CO) in 24- or 48-well plates and HiPerfect Transfection Reagent (Qiagen), respectively 4.5 or 2 µl according to the manufacturer's protocol. The cells were incubated for 48 or 72 hours at which time in vitro lipogenesis was assessed in cells plated on 48-well plates and RNA were collected.
Growth protocol Primary adipocyte culture for in vitro studies was obtained from subcutaneous WAT from healthy non-obese men and women (BMI <30 kg/m2) undergoing cosmetic liposuction. The stroma vascular fraction (SVF) cells were isolated. The precursor cells obtained from separate individuals were not mixed. Part of plastic-adherent SVF cells were directly plated (30,000-50,000 cells/cm2) and differentiated to adipocytes as described. The degree of differentiation was controlled under the microscope as accumulation of lipids, and response to insulin in lipogenesis assay was evaluated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA were amplified and biotin labeled using the Whole Transcript (WT) Sense Target Labeling Protocol.
 
Hybridization protocol From non-degraded high-quality total RNA we prepared and hybridized biotinylated complementary RNA to Gene 2.1 ST Arrays, and then washed and stained slides using standardized protocols (Affymetrix Inc., Santa Clara, CA).
Scan protocol Arrays were scanned using standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA).
Description 212_siSLC25A10_C07
Data processing These arrays were pre-processed with RMA, which includes normalization, summarization of probes to probesets, and background correction.
Analysis Method: rma-gene-full
Analysis Software: Expression Console
Data Transformation: log2
Annotations: hg19/GRCh37
NetAffx build: 33
 
Submission date Feb 09, 2017
Last update date Jun 29, 2017
Contact name Ingrid Dahlman
E-mail(s) [email protected]
Organization name Karolinska
Street address Karolinska Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platform ID GPL17692
Series (2)
GSE94751 Global transcriptome profiling identifies KLF15 and SLC25A10 as regulators of adipocytes insulin sensitivity in obese women [siRNA]
GSE94753 Global transcriptome profiling identifies KLF15 and SLC25A10 as regulators of adipocytes insulin sensitivity in obese women

Data table header descriptions
ID_REF
VALUE Log2 transformed RMA expression value

Data table
ID_REF VALUE
16650001 1.43
16650003 1.97
16650005 0.84
16650007 2.9
16650009 0.59
16650011 0.74
16650013 4.47
16650015 3.65
16650017 2.52
16650019 3.07
16650021 4.03
16650023 2.36
16650025 2.71
16650027 3.02
16650029 1.98
16650031 1.54
16650033 0.82
16650035 1.63
16650037 0.87
16650041 4.2

Total number of rows: 53617

Table truncated, full table size 727 Kbytes.




Supplementary file Size Download File type/resource
GSM2482093_212_siSLC25A10_C07.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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