|
| Status |
Public on Jun 01, 2009 |
| Title |
Affected2 |
| Sample type |
RNA |
| |
|
| Source name |
EBV-transformed cell line of lymphoblasts extrated from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
Immortalized lymphoblastoid cell line
|
| Treatment protocol |
No treatment
|
| Growth protocol |
RPMI-1640 medium (Invitrogen, Inc., Carlsbad, CA), containing 2 mmol/L L-glutamine, 100 ug/ml streptomycin, and 10% fetal calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent (Invitrogen, Inc.)
|
| Label |
Biotin-Labeled cRNA
|
| Label protocol |
Illlumina® TotalPrep RNA amplification kit, manufactured by Ambion, Inc (Austin, TX) according to the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
Refer to the Illumina Gene Expression System Manual
|
| Scan protocol |
Refer to the Illumina Gene Expression System Manual
|
| Description |
1825389067B_Affected2
Comparison of genome-wide expression in EBV-transformed lymphoblastoid cell lines of patients and controls
|
| Data processing |
Raw data for each sample was output using the Illumina BeadStudio. Default data transformation to set measurements below 0.01 to 0.01 was applied. Default settings were used for experiment normalization which included chip normalization to 50th percentile and gene normalization to the median
|
| |
|
| Submission date |
Dec 07, 2007 |
| Last update date |
Dec 29, 2008 |
| Contact name |
Yelena Bykhovskaya |
| Organization name |
Cedars-Sinai Medical Center
|
| Street address |
8700 Beverly Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL6106 |
| Series (1) |
| GSE9822 |
Expression profiling in lymphoblastoid cell lines of family with maternally inherited non-syndromic hearing loss. |
|
