|
| Status |
Public on Oct 13, 2017 |
| Title |
emu_feather_1_sample_E6FL |
| Sample type |
SRA |
| |
|
| Source name |
emu_feather placode tract epidermis
|
| Organism |
Dromaius novaehollandiae |
| Characteristics |
developmental stage: Hamburger and Hamilton Stage 33/34
tissue: feather placode tract epidermis
|
| Treatment protocol |
Skin samples were dissected and placed in a minimal amount of 10 mg/mL dispase and incubated at 4 degrees celsius for 13 hours to permit clean separation of epidermis.
|
| Growth protocol |
Bird eggs were incubated with rotation in a G.Q.F 1502 Sportsmen Incubator at 37.8 degrees celsius (chicken) or 35.5 degrees celsius (emu)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a Qiagen Rneasy kit.
polyA enriched strand-specific library was constructed using a proprietary method developed by the Yale Center for Genome Analysis
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA
reads mapped to chicken or emu genome using Tophat2 version 2.0.6. using default parameters and setting the -GTF option
read counts generated using HTSEQ version 0.5.3p9 implemented in python version 2.7.2. We set the option requiring reads to map to the correct strand, and resolved issues with multiple mappings using the option "intersection-nonempty".
TPM (transcript per million) relative expression values were calculated for all genes using the following method: Wagner GP, Kin K, Lynch VJ. 2012. Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples. Theor Biosci. 131:281-285.
We applied a scaling factor to all emu TPM values (.55x) to account for differences in the number of annotated genes. This value was calculated using the method outlined here: Musser, J. M. & Wagner, G. P. Character trees from transcriptome data: Origin and individuation of morphological characters and the so-called ‘species signal’. J Exp Zool (Mol Dev Evol) 324, 588–604 (2015).
Genome_build: Chicken reads were mapped to ensembl WASHUC2 release version 68. Emu reads were mapped to a preliminary version of the Emu genome generated by the Alan Baker Lab at the University of Toronto.
Supplementary_files_format_and_content: concerted_evolution_scaled_tpms.txt is a tabular format text file that contains the final scaled TPM values for all chicken and emu one-to-one orthologs that were used for analyses.
|
| |
|
| Submission date |
Oct 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jacob Musser |
| E-mail(s) |
[email protected]
|
| Organization name |
European Molecular Biology Laboratory
|
| Street address |
Meyerhofstrasse 1
|
| City |
Heidelberg |
| ZIP/Postal code |
69117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22596 |
| Series (1) |
| GSE89040 |
Pervasive concerted evolution in gene expression shapes cell type transcriptomes |
|
| Relations |
| BioSample |
SAMN05933638 |
| SRA |
SRX2263389 |
