|
| Status |
Public on Aug 22, 2017 |
| Title |
mNSC_E14.5_Cre_sample_8 |
| Sample type |
RNA |
| |
|
| Source name |
embyronic day 14.5 cortex
|
| Organism |
Mus musculus |
| Characteristics |
age: E14.5
cell type: neural stem cell
transduction: adenoviral-Cre recombinase
|
| Treatment protocol |
mNSCs were treated with adenoviral-GFP or adenoviral-Cre-recombinase available from VectorBiolabs.100MOI was used for infection. 24 hours post-infection, cells were washed and cultured in complete media.
|
| Growth protocol |
mNSCs were cultured following the NeuroCult protocol In vitro proliferation and differentiation of mouse neural stem and progenitor cells using NeuroCult.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cells following the Qiagen Rneasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE). Hybridization targets were prepared with Ambion WT Expression Kit Life Technologies/Ambion (Grand Island, NY) and Affymetrix GeneChip WT Terminal Labeling Kit from total RNA.
|
| |
|
| Hybridization protocol |
Product generated from the label protocol was hybridized onto the Affymetrix GeneChip Mouse Gene 2.0 ST array in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
|
| Scan protocol |
Arrays were scanned with Affymetrix GeneChip® Scanner 7G according to Affymetrix GeneChip protocols.
|
| Description |
WT
|
| Data processing |
Affymetrix Expression Console was used for data processing and normalization. RMA normailzation was applied
|
| |
|
| Submission date |
Oct 17, 2016 |
| Last update date |
Aug 22, 2017 |
| Contact name |
Hai Yan |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
Pathology
|
| Lab |
Yan Lab
|
| Street address |
199B-MSRB, Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL18233 |
| Series (1) |
| GSE88828 |
Effects of IDH1-R132H on mouse neural stem cells |
|
