|
| Status |
Public on May 15, 2017 |
| Title |
HSCs-PBS-repl2 |
| Sample type |
RNA |
| |
|
| Source name |
HSCs FACS-sorted from PBS-injected mice.
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse hematopoietic stem cells (HSC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation was performed using ARCTURUS® PicoPure® RNA Isolation Kit (Life Technologies, Invitrogen) according to the manufacturer’s instructions. DNAse treatment was performed using RNAse-free DNAse Set (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Hybridization performed on a Affymetrix Fluidics Station
|
| Scan protocol |
Array scanning and raw data processing were done using GCOS software (Affymetrix)
|
| Description |
Gene expression data from mouse HSCs
|
| Data processing |
CEL files were imported to R statistical package version 3.3.1 using R oligo package version 1.36.1. Microarray data was subjected to RMA normalization. Significance analysis was performed using the bioconductor package LIMMA version 3.28.20
|
| |
|
| Submission date |
Oct 07, 2016 |
| Last update date |
May 15, 2017 |
| Contact name |
Sandro Altamura |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Heidelberg
|
| Department |
Dep. Pediatric Hematology, Oncology and Immunology
|
| Street address |
Im Neuenheimer Feld 350
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE87763 |
Exit from HSC dormancy is controlled via vitamin A/retinoic acid (I) |
| GSE87814 |
Exit from HSC dormancy is controlled via vitamin A/retinoic acid |
|
