|
| Status |
Public on Sep 18, 2016 |
| Title |
Male CP_sham_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Male choroid plexus_sham
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: choroid plexus
experimental procedure: Sham-operated
gender: Male
|
| Treatment protocol |
Wistar rats were housed under a 12-h light, 12-h dark cycle, with food and water ad libitum during the course of the experiment. Female and male rats (2 months) were either ovariectomized or orchidectomized. Two weeks after surgery, castrated animals were anesthetized with a mixture of ketamine and medetomidine. Cerebral hemispheres were separated and both lateral ventricular CP removed and immediately frozen in liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer’s instructions. RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies), and RNA integrity was assessed by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). All RNA samples used in this study had an A260/A280 ratio of.1.9
|
| Label |
Cy3
|
| Label protocol |
Pools of RNA samples from three animals were biological replicates. Equal amounts of RNA extract (200ng) from the 3 pools of sham or 3 pools of GDX animals, in a total of three biological replicates, were amplified and Cy-3-labeled using the Low Input Quick Amp Labeling kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridizations were carried out on an Agilent-based microarray platform using custom-designed whole genome Rat GE 4x4K v3 microarrays, and arrays were scanned with an Agilent microarray scanner (Agilent Technologies).
|
| Scan protocol |
Scanned images were analyzed by Feature extraction software (Agilent Technologies) using GE1_105_Dec08 protocol.
|
| Description |
CP gene expression in sham males
|
| Data processing |
The signal intensity was normalized between microarrays by centering the median of the signal distribution using BRB-ArrayTools v3.8.1 (http://linus.nci.nih. gov/BRB-ArrayTools.html).
|
| |
|
| Submission date |
Sep 17, 2016 |
| Last update date |
Sep 18, 2016 |
| Contact name |
Telma Quintela |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Beira Interior
|
| Department |
Health Sciences Research Centre
|
| Street address |
Av. Infante D. Henrique
|
| City |
Covilhã |
| ZIP/Postal code |
6200-506 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE87045 |
Sex hormone responsive genes in rat choroid plexus |
|
