|
| Status |
Public on Sep 15, 2016 |
| Title |
LNCaP(BHC80-1)_1 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP(BHC80-1)
|
| Organism |
Homo sapiens |
| Characteristics |
tumor subtype: Adenocarcinoma
cell line: LNCaP
treatment: BHC80-1 overexpression
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction protocol not provided.
|
| Label |
Cy3
|
| Label protocol |
The quality of the RNA samples was checked with the Agilent 2100 Bioanalyzer and NanoDrop ND-2000 UV-VIS spectrophotometer. Only samples with RNA Integrity Number (RIN) ≥8.0, A260/280 OD values between 1.8 and 2.0 and an A260/A230 OD value of 2.0 were used for one-color labelling using Agilent’s One-Colour Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling v6.0 (Agilent Technologies, Santa Clara, CA). Total RNA (100 ng) was used to generate cyanine-3-labeled cRNA.
|
| |
|
| Hybridization protocol |
cRNAs were hybridized on Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray (AMDID 039494).
|
| Scan protocol |
Arrays were scanned with an Agilent DNA Microarray Scanner at a 3 µm scan resolution
|
| Description |
SAMPLE 4
|
| Data processing |
Data were processed with Agilent Feature Extraction 11.0.1.1. Processed signals were quantile normalized with Agilent GeneSpring 12.0. Raw and normalized data can also be found in the file AgilentHumanv2GE_20Nov14.txt available on the series record.
|
| |
|
| Submission date |
Sep 14, 2016 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Shawn Anderson |
| E-mail(s) |
[email protected]
|
| Organization name |
Vancouver Prostate Centre
|
| Lab |
Laboratory for Advanced Genome Analysis
|
| Street address |
2660 Oak Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6H3Z6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE86942 |
RNA Splicing of the BHC80 gene Facilitates Neuroendocrine Prostate Cancer Progression |
|
| Relations |
| Reanalyzed by |
GSE113533 |
