|
| Status |
Public on Feb 14, 2017 |
| Title |
F4/80lowPMO_SL3261_day5_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
F4/80 low peritoneal macrophages from Salmonella Typhimurium (SL3261) infected C57BL/6 females 5 d.p.i.
|
| Organism |
Mus musculus |
| Characteristics |
tissue: peritoneal cavity
strain: C57BL/6
Sex: female
cells: F4/80 low macrophages
infection: SL3261
|
| Treatment protocol |
Animals were infected with 200 L3 H.polygyrus and 9 days post infection co-infected with 3x10^4 cfu Salmonella Typhimurium (SL3261) and peritoneal macrophages analysed 5 days later (Day 14 p. H.polygyrus infection). As control uninfected (naïve) or single H.polygyrus / Salmonella Typhimurium infected animals were used. Whole peritoneal cells were isolated by peritoneal lavage and F4/80 high / low macrophages sorted by flow cytometry (CD19-TCRb-, SiglecF-, Ly6G-, CD11b+, CD115+, F4/80 high / low)
|
| Growth protocol |
C57BL/6 female mice, aged approx 7-10 weeks at the start of the experiment housed in IVC cages under SPF conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using the RNeasy Mini Kit (Qiagen) as described by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Total RNA (10 ng) were converted to cDNA, fragmented and biotinylated using the GeneChip WT Pico Reagent kit (Affymetrix) following the manufacturer's instruction.
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| |
|
| Hybridization protocol |
5.5 ug of fragmented and labeled sscDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from bacterial infection induced, recruited, blood monocyte derived macrophages
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.6 with the following options: probesets were quantile normalised and RMA background correction applied. Probesets were summarised to genes by calculating the means (log 2).
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| |
|
| Submission date |
Aug 18, 2016 |
| Last update date |
Feb 14, 2017 |
| Contact name |
Dominik Andreas Rückerl |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Manchester
|
| Department |
Faculty of Biology, Medicine and Health
|
| Street address |
Oxford Road
|
| City |
Manchester |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE85805 |
Comparison of F4/80 high and low peritoneal macrophages in H.polygyrus - Salmonella Typhimurium co-infected mice |
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