|
| Status |
Public on Aug 01, 2009 |
| Title |
GVGdvl4mock2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis rosette leaves.
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Material was derived from mock treated fully expanded Arabidopsis rosette leaves 10hrs post-treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the leaf samples using TRIzol reagent (Invitrogen) and purified using the RNeasy Midi Kit (Qiagen) according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
cDNA synthesis and labeling was completed according to chip manufacturer's instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was completed according to chip manufacturer's instructions (Affymetrix) using an Affymetrix GeneChip Hybridization Oven 640 and GeneChip Fluidics Station 450.
|
| Scan protocol |
Microarrays were scanned and data acquired using an Affymetrix GeneChip Scanner 3000 driven by GCOS 1.2 software (Affymetrix).
|
| Description |
Plants were grown in artificial greenhouse potting media in standard growth chambers at 22C and 16hr days. Plants were treated when they reached the six expanded rosette leaf stage (vegetative) with mock treatment or 30um dex in water. At 10hrs post-treatment, the largest rosette leaf was collected from 20 plants. These leaves were pooled and were considered one biological replicate.
|
| Data processing |
Data were processed using dChip software (www.dchip.org).
|
| |
|
| Submission date |
Sep 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Clayton T Larue |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Missouri, Columbia
|
| Department |
Dept of Biological Sciences
|
| Lab |
John Walker Lab
|
| Street address |
304 Life Sciences Bldg
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE8975 |
Global expression profiling of Arabidopsis plants overexpressing a member of the DEVIL gene family. |
|
| Relations |
| Reanalyzed by |
GSE119083 |
