|
| Status |
Public on Jul 22, 2009 |
| Title |
Carrier biological rep3 (Glyphosate) |
| Sample type |
RNA |
| |
|
| Source name |
Leaf, surfactant (preference, 0.25%) 24h post tretment
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
No visible symptoms of injury observed
|
| Treatment protocol |
14 days old plants were sprayed with surfactant and herbicide solutions in a RIC spray chamber (RC-5000-100EP)
|
| Growth protocol |
Seeds of A. thaliana, col 0 were stratified at 3-4C for 2-4 days and then grown under 22-23C, 16h photoperiod and 175-210 micromol per sec light intensity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Triazol extraction of total RNA and subsequent purification with Rneasy mini spin columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip scanner 3000 at 570 nm.
|
| Description |
none
|
| Data processing |
Intensities of the fluorescence emission were captured and array image was visually screened for signal artifacts
|
| |
|
| Submission date |
Aug 31, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Malay Das |
| E-mail(s) |
[email protected]
|
| Phone |
5417544315
|
| Fax |
5417544799
|
| Organization name |
National Research Council, US-EPA
|
| Department |
EEB
|
| Street address |
200 SW 35th STreet
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97333 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE8927 |
Global expression profiling to study the effect of glyphosate herbicide treatment on Arabidopsis thaliana |
|
| Relations |
| Reanalyzed by |
GSE119083 |
